Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases.
These death signals finally cause the degradation of chromosomal DNA by activated DNase.
A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1,2).
The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4).
Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (5).
Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei (1-4).
Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000.
50ug of murine kidney tissue lysate with anti-CAD (I17) at 1:500 Optimal dilutions to be determined by the researcher.
Positive Control: Mouse lung tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：A 40kD band should be detected. Western blot analysis of 50ug of mouse kidney tissue lysate with anti-CAD (I17) at 1:500 dilution.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Peptide corresponding to amino acids 205 to 222 of murine CAD (1).
