Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases.
These death signals finally cause the degradation of chromosomal DNA by activated DNase.
A human DNA fragmentation factor (DFF) was identified recently which is cleaved by caspase-3 during apoptosis (1).
Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase (2,3).
Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2,3).
Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death (4).
Applications:Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: paraffinOptimal dilutions to be determined by the researcher.
Positive Control: Murine lung tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes mouse ICAD. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to mouse ICAD at N-terminal.
