MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains (1).
Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2 (1).
MADD shares 98% identity with DENN (for differentially expressed in neoplastic vs.
normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3) (2, 3).
MADD has greater than 94% overall identity to a GDP/GTP exchange protein Rab3-GEP (4, 5).
MADD is 87% identical to KIAA0358, a brain protein of unknown function (4).
Identification of MADD as a component of the TNFR1 signaling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western blot: 1:250-1:500 (200 to 220kD bands detected)
Optimal dilutions to be determined by researcher.
Positive Control: Whole cell lysate from HeLa or NIH3T3 cells.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes MADD. Species crossreactivity: Human and mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to amino acids 1570-1588 of human MADD (1). The sequence of immunogenic peptide is identical to that of DENN and differs by one amino acid with rat GDP/GTP exchange protein Rab3-GEP (2,5).
