84-1014-66　FLIP, NT (FLICE Inhibitory Protein) 100ug　F4550-05

特徴

• Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
• Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family.
• Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family.
• FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively1,2.
• The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7.
• FLIP contains two death effector domains (DEDs) and a caspase-like domain.
• FLIP interacts with adapter protein FADD and caspase-8 and –10, and potently inhibits apoptosis induced by all known death receptors.
• Four splice variants of c-FLIPs have been identified and termed FLIPa, b, g, and d, respectively.
• Applications:Suitable for use in Flow Cytometry, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot and Immunocytochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1-2ug/mlImmunohistochemistry (paraffin): 5ug/mlImmunocytochemistry: 5ug/mlImmunofluorescence: 20ug/mlOptimal dilutions to be determined by the researcher.
• Positive Controls:HeLa Cell Lysate Mouse Liver Tissue LysateJurkat Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.