Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.
Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family.
FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively (1,2).
The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT (3-8).
FLIP contains two death effector domains (DEDs) and a caspase-like domain.
FLIP interacts with adapter protein FADD and caspase-8 and –10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.
This polyclonal antibody can be used for the detection of FLIP by Western blot at 1:500 to 1:1000 dilution.
NIH/3T3 whole cell lysate can be used as positive control and a 55kD band can be detected.
FLIP has short form (FLIPS) and long form (FLIPL) and this antibody recognizes the FLIPL only.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：A 55kD band can be detected. FLIP has short form (FLIPS) and long form (FLIPL) and recognizes the FLIPL only.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Peptide corresponding to amino acids 449 to 465 of mouse FLIPL/CASHa (2,6).
