Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases.
A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3).
Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (3).
Caspase-9 is also activated by granzyme B and CPP32 (1,2).
Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3).
Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFa, TRAIL, anti-CD-95, FADD, and TRADD (4).
Caspase-9 is expressed in a variety of human tissues (1,2).
This polyclonal antibody can be used for detection of caspase-9 by Western blot at 1:1000 to 1:2000 dilution.
Whole cell lysate from HeLa cells can be used as positive control and a 46kD band should be detected.
It has no cross response to other members in caspase family.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:2000 Optimal dilutions to be determined by the researcher.
Positive Control: Whole cell lysate from HeLa cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：A 46kD band should be detected. It has no cross response to other members in caspase family.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acids 41 to 56 of human caspase-9 (1-3).
