Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms.
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas.
Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family.
A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx1,2.
Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway.
Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways (1,2).
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting: 1:500 to 1:1000.
HeLa whole cell lysate can be used as positive control and a 120kD major band can be detected
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Daxx (CT). The sequences of immunogenic peptide are identical among human, monkey and mouse origins of Daxx1,2, and the antibody recognizes Daxx from all these species.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acids 722 to 740 at C-terminus of human Daxx 1,2.
