Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors.
TRAIL/Apo2L is a new member of the TNF family.
DR4 was recently identified as the receptor for TRAIL.
A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently (1-7).
Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells.
DR5 binds to TRAIL and mediates TRAIL induced cell death.
Overexpression of DR5 induces apoptosis and activates NF-kB.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:250-1:500.
HeLa whole cell lysate can be used as a positive control
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.01% sodium azide, 40% glycerol.
Specificity：Recognizes human DR5 (Apo2, Trail-R2, Trick2, Killer, Death Receptor5). Does not crossreact with DR4. Species crossreactivity: Human and mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to amino acids 388-407 of human DR5 precursor (1,2).
