Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms.
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas.
A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4)1.
The ligand for this novel death receptor has been identified and termed TRAIL2,3, which is a new member in the TNF family.
DR4 is also called TRAIL receptor-1 (TRAIL-R1)4.
DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus.
Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-kB activation in many tissues and cells.
This polyclonal antibody can be used for detection of DR4 by Western blot at 1:500 to 1:1000 dilution.
HeLa whole cell lysate can be used as positive control and a 57kD band can be detected.
Western blot analysis of 50 ug of total cell lysate from HeLa cells with anti-DR4 (NT) at 1:1000 dilution.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control: HeLa whole cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：A 57kD band can be detected.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acid 1 to 20 of human DR4 mature protein.
