Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms.
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas.
A novel cell death receptor was recently identified by several groups independently and designated DR3, Wsl-1, Apo-3, TRAMP and LARD.
The ligand for this novel death receptor has been defined as TWEAK, also termed Apo3L.
DR3 is highly expressed in the tissues enriched in lymphocytes including PBL, thymus and spleen.
Like TNFR1, DR3 induces apoptosis and NF-kB activation.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended DilutionS:Western Blot: 1:500-1:1000.
A band at ~59kD can be seen with Jurkat whole cell lysate
Optimal dilutions to be determined by researcher.
Positive Control:Jurkat whole cell lysate.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human DR3 at ~59kD. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa25-40 of human DR3 precusor.
