Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms.
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas.
A novel cell death receptor was recently identified by several groups independently and designated DR3, Wsl-1, Apo-3, TRAMP and LARD1-5.
The ligand for this novel death receptor has been defined as TWEAK, also termed Apo3L.
DR3 is highly expressed in the tissues enriched in lymphocytes including PBL, thymus and spleen.
Like TNFR1, DR3 induces apoptosis and NF-kB activation.
This polyclonal antibody can be used for detection of DR3 expression by Western blot at 1:500 to 1:1000 dilution, and in Immunocytochemistry Jurkat whole cell lysate can be used as positive control and a 59kD band should be detected.
Applications:Suitable for use in Western Blot, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control: Jurkat whole cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：A 59kD band should be detected.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acids 59 to 77 in extracellular domain (ED) of human DR3 precusor1,2.
