Members of the glial cell line-derived neurotrophic factor (GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation.
A new member of the GDNF family was recently identified and designated persephin (1).
Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb (2).
Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3 (3-7).
GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.
Applications:Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlImmunohistochemistry (paraffin): 5ug/mlImmunofluorescence: 5ug/mlOptimal dilutions to be determined by the researcher.
Antibody Blocking Peptide:G2033-50P
Positive Control:Mouse kidney, liver or heart tissue lysates.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Highly Purified
Purity：Purified by DEAE chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes mouse GFR alpha-3 (IN). Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to 14aa near the center of mouse GFR alpha 3.Located within the last 50 amino acids of GFR alpha 3.
