84-1014-48　GFR alpha2, IN (Glycosylphosphadidylinositol linked Protein, GDNFRb, Ret ligand 2, RETL2) 100ug　G2033-25

特徴

• Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN) (1), play key roles in the control of vertebrate neuron survival and differentiation.
• Physiological responses to NTN require the presence of a novel glycosylphosphadidylinositol linked protein NTNRa, which is a cell surface receptor for NTN (2,3).
• The cDNAs encoding NTNRa from human, rat, chicken, and mouse have been cloned recently (2-7).
• NTNRa was also termed GDNFRb, Ret ligand 2 (RETL2) or TGF-b-related neurotrophic factor receptor 2 (TrnR2) (4-7) and nominated as GFRa-2 recently (8).
• GFRa-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF.
• Thus, NTN, GFRa-2, and the Ret PTK form a complex to transduce NTN signal and to mediate NTN function.
• This polyclonal antibody can be used for detection of GFRa-2 expression by Western blot at 1:1000 to 1:2000 dilution.
• HeLa whole cell lysate can be used as positive control and a 52kD band should be detected.
• The amino acid sequences of immunogenic peptide are identical to those of mouse and rat and the antibody recognizes GFRa-2 of human, mouse, and rat origins.
• Antibody blocking peptide: G2033-25P.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:2000Optimal dilutions to be determined by the researcher.
• Positive Control: HeLa whole cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.