Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system.
Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRa, which is a cell surface receptor for GDNF (1,2).
The cDNAs encoding GDNFRa from human, rat, chicken and mouse have been cloned recently (1-5).
GDNFRa was also termed Ret ligand 1 (RETL1) or TGF-b-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRa-1 recently (5-7).
GFRa-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK).
Thus, GDNF, GFRa and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.
This polyclonal antibody can be used for detection of GFRa-1 by Western blot.
The amino acid sequence of immunogenic peptide is identical to those of mouse and rat and the antibody recognizes GFRa-1 from human, mouse and rat origins.
Antibody blocking peptide: G2033-01P.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human GFR alpha-1 (IN).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acids 369 to 382 of human GFRa-1 (1).
