CCR8 is one of the chemokine receptors that are required as coreceptors for HIV infection.
The genes encoding human and murine CCR8 were cloned and designated TER1, CKR-L1, and ChemR1 (1-4).
The encoded seven transmembrane protein was identified as the receptor for human CC chemokine I-309 and renamed CCR8.
Recently, CCR8 was found to serve as a coreceptor for diverse T-cell tropic, dual-tropic and macrophage-tropic HIV-1 strains (5).
CCR8 mediates CC chemokine I-309 induced monocyte chemoattraction and HIV-1 envelope fusion and virus infection, which can be prevented by the CCR8 ligand I-309.
CCR8 is expressed in spleen, thymus and T lymphoblastic cell lines.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1,000Optimal dilutions to be determined by the researcher.
Positive Control:Human spleen tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human CCR8 (TER1, CKR-L1, ChemR1, Chemokine Receptor 8, CC-CKR-8). A ~50kD band can be detected.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acids 183 to 201 of human CCR8, which locate in the second extracellular loop (1-3).
