84-1014-41　SIRP alpha (SIRPA, Tyrosine-Protein Phosphatase Non-receptor Type Substrate 1, SHP Substrate 1, SHPS-1, Brain Ig-like Molecule with Tyrosine-based Activation Motifs, Bit, CD172a, CD172 Antigen-like Family Member A) 100ug　S1013-87

特徴

• Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death.
• SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors.
• A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7).
• SIRPa is a new gene family containing at least fifteen members.
• SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8).
• SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion.
• It plays a general role in different physiological and pathological processes (1,2,5,8).
• Applications:Sutiable for use in ELISA, Immunocytochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5-1ug/ml.
• Predicted molecular weight: 75-110kD
• Immunocytochemistry: 1ug/mlOptimal dilutions to be determined by researcher.
• Positive Control:Whole cell lysate from THP-1 cells.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.