84-1014-40　Dok, p62, CT (Dok-1, Docking Protein, Downstream of Tyrosine Kinase, FRIP) 100ug　D8080

特徴

• Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases.
• Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli.
• A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories.
• Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP).
• p62dok represents a new family with very recently identified p56dok.
• Human DOK1 is a 481 amino acid protein.
• Docking proteins are substrates of tyrosine kinases and function in the recruitment and assembly of specific signal transduction molecules.
• Here we found that p62dok family members act as substrates for the c-Ret receptor tyrosine kinase.
• In addition to dok-1, dok-2, and dok-3, two new family members, dok-4 and dok-5 have been identified that can directly associate with Y1062 of c-Ret.
• Dok-4 and dok-5 constitute a subgroup of dok family members that is coexpressed with c-Ret in various neuronal tissues.
• Activated c-Ret promotes neurite outgrowth of PC12 cells; for this activity, Y1062 in c-Ret is essential.
• c-Ret/dok fusion proteins, in which Y1062 of c-Ret is deleted and replaced by the sequences of dok-4 or dok-5, induce ligand-dependent axonal outgrowth of PC12 cells, whereas a c-Ret fusion containing dok-2 sequences does not elicit this response.
• Dok-4 and dok-5 do not associate with rasGAP or Nck, in contrast to p62dok and dok-2.
• Moreover, dok-4 and dok-5 enhance c-Ret-dependent activation of mitogen-activated protein kinase.
• Applications: Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:2000.
• A 62kD band should be detected.
• Optimal dilutions to be determined by the researcher.
• Positive Control: Whole cell lysate from Jurkat cells.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.