STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors.
After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes.
Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile (1,2).
STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g, and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1) (3).
STAT1 has two forms, the 91kD STAT1a and the 84kD STAT1b, which are encoded by the same gene with splicing variant (4).
Applications:Suitable for use in Western Blot, ELISA, Immunofluorescence, Immunocytochemsitry, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ugImmunoprecipitation: 2-4ugImmunocytochemistry: 10ug/mlImmunohistochemistry: 2.5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control: Whole cell lysate from HeLa, Jurkat, or A431 cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human STAT 1a. A 90kD band can be detected. No reaction to the 84kD STAT1b. Species crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IC IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a 38 amino acids fragment within the last 50aa of human STAT1a. The sequences differ from the murine corresponding sequences by four amino acids. Species sequence homology: porcine.
