84-1014-37　IKK alpha, C3 (IkB Kinase) 100ug　I3000-10

特徴

• Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses.
• NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS.
• NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity.
• The long-sought IkB kinase (IKK), which phosphorylates IkB, and mediates IkB degradation and NF-kB activation, was recently identified by several laboratories (1-5).
• IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKa and IKKb).
• IKKa and IKKb interact with each other and both are essential for the NF-kB activation.
• IKKa specifically phosphorylates IkB-a.
• IKKa is expressed in a variety of human tissues.
• This polyclonal antibody can be used for detection of IKKa by Western blot at 1:500 to 1:1000 dilution.
• Whole cell lysate from Jurkat cells can be used as positive control and an 85kD band should be detected.
• It has no cross response to IKKb or IKKg.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Positive Control: Whole cell lysate from Jurkat cells
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.