Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses.
NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa and bacteria product LPS.
NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity.
The long-sought IkB kinase (IKK), which phosphorylates IkB, and mediates IkB degradation and NF-kB activation, was recently identified by several laboratories (1-5).
IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKa and IKKb).
IKKa and IKKb interact with each other and both are essential for the NF-kB activation.
IKKa specifically phosphorylates IkB-a.
IKKa is expressed in a variety of human tissues.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Positive Control: Whole cell lysate from HeLa cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes IKK alpha, C2. An 85kD band should be detected. It has no cross response to IKKb or IKKg.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acids 699 to 715 of human IKKa (1,2), which differs from corresponding murine sequence by one amino acid.
