Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses.
NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS.
NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity.
The long-sought IkB kinase (IKK), which phosphorylates IkB, and mediates IkB degradation and NF-kB activation, was recently identified by several laboratories.
IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKa and IKKb).
IKKa and IKKb interact with each other and both are essential for the NF-kB activation.
IKKa specifically phosphorylates IkB-a.
IKKa is expressed in variety of human tissues.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Whole cell lysate from HeLa or Jurkat cells.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.
Specificity：Detects an 85kD band. Does not crossreact with IKKb or IKKg.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to aa716-734 of human IKKa (1,2). Differs from corresponding murine sequence by four amino acids.
