The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1).
IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment.
The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kB inducing kinae (NIK) and of NF-kB, recruits to the IL-1R complex through IL-1RAcP (2).
IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP (3,4).
Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response.
IL-1RAcP is expressed in many tissues.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml Detects a band at ~63kD with whole HeLa lysatesImmunocytochemistry: 5ug/ml.
Optimal dilutions to be determined by the researcher.
Positive Control:HeLa whole lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Interleukin 1 Receptor AcP at ~63kD. No crossreactivity to other members in the IL-1 receptor family.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 22 amino acids near the carboxy terminus of human Interleukin 1 Receptor AcP. Species Sequence Homology: rat; 91%, mouse; 86%
