The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor 1 (IL-1R1) and IL-1 receptor accessory protein (IL-1RAcP).
IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response.
A novel member in the IRAK/Pelle family was recently identified and designated IRAK2.
Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation.
IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation.
Members in the IRAK/Pelle family play a central role in IL-1R- and TLR-mediated inflammatory response.
IRAK2 is expressed in a variety of human tissues.
Applications:Suitable for use in ELISA, Western Blot., Immunohistochemistry Other applications not tested.
Recommended Dilution:Western Blot: 1:250-1:500 (a 65kD band should be detected)Optimal dilutions to be determined by the researcher.
Positive Control:Whole cell lysate from HeLa or K562 cells
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.01% sodium azide, 40% glycerol.
Specificity：Recognizes IRAK2. A 65kD band should be detected. IRAK2 has no crossreactivity to IRAK.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to aa546-564 of human IRAK2.
