Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses.
NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa and LPS.
A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2).
IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3).
IRAK mediates a signaling cascade leading to NF-kB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:500Immunoprecipitation: 2-4ug per sampleOptimal dilutions to be determined by the researcher.
Positive Control:THP-1 or HeLa cells can be used.
80kD band should be detected in the lysate from non-activated cells.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes 80kD band should be detected in the lysate from non-activated cells. It has no crossreactivity to IRAK2.Species Crossreactivity: Human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to amino acids 700 to 712 of human IRAK (1).
