Applications:Suitable for Immunoblotting (western or dot blot), ELISA and Immunohistochemistry.
Recommended Dilutions:ELISA: Assayed against 1ug of goat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 min.
at room temperature.
A working dilution of 1:2000 to 1:8000 of the reconstitution concentration is suggested
Optimal dilutions to be determined by the researcher.
Storage Conditions:Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
仕様
Size：1mg
Host：donkey
Source Antibody：goat
Grade：Affinity Purified
Purity：Prepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by solid phase adsorption.
Specificity：No reaction was observed against chicken, guinea pig, hamster, equine, mouse, rabbit and rat serum proteins. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-donkey serum, goat IgG and goat serum.
