Borrelia burgdorferi is a gram-negative spirochete that is a causative agent of lyme disease in humans.
Applications:Suitable for use in FLISA, Immunohistochemistry and Immunoflourscence.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:100-1:1000Immunohistochemistry: 1:100-1:1000 on frozen section or formalin-fixed paraffin-embedded sections after antigen retrieval
Immunoflourscence: 1:100-1:1000Optimal dilutions to be determined by the researcher.
Molar Flourescein / Antibody Protein Ratio:3-7
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months at -20°C after receipt.
Reconstitute with sterile dH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：goat
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder with BSA. Contains no preservatives. Labeled with fluorescein isothiocyanate (FITC). Molar ratio: 4.4:1 Reconstitute with 1ml sterile ddH2O, 0.09% sodium azide. Additional dilutions can be made using PBS.
Specificity：Highly specific for Borrelia burdorferi. Cross-reactivity with other Borrelia species has been minimized through extensive affinity adsorption. Has not been tested for crossreactivity to treponemes.
Isotype：IgG
Calc Applications Abbrev：FC FLISA IHC
Immunogen：Heat-killed whole cells of Borrelia burdorferi.
