Applications: Suitable for use in ELISA, Western Blotting, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100 (1.0ug/ml) Western Blotting: 1:50 (2.0ug/ml )Immunohistochemistry: 1:10 (10ug/ml )Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O or PBS prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Grade：Affinity Purified
Purity：≥ 99% Purified by affinity chromatography. No contaminants detected. Single band by SDS-PAGE, IEP, and/or RID.
Form：Supplied as a lyophilized powder. Contains no stabilizers or preservatives. Labeled with horseradish peroxidase (HRP)
Specificity：Affinity adsorbed to limit crossreactivity with B. hermsii and B. anserina, B. coriaceae.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Heat-killed whole cells of Borrelia burdorferi.
