In normal tissues and in a variety of myeloproliferative disorders, myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO.
It is readily detected in myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythro-leukemia, myeloblastomas, and other hematopoietic disorders.
Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive.
Applications:Suitable for use in ELISA and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: Exhibits a P-ANCA patternELISA: 1:100Optimal dilution determined by the researcher.
Autoantibody Activity:Anti-MPO Activity by ELISA: As reported (lot-specific).
Current lot value: 96EU/mlInterpretation of Results: <4EU/ml: Negative; 4.1-5.9EU/ml: Equivocal; >6EU/ml: Positive
Other Autoantibody Activity in the Serum:The serum exhibits no reactivity at a dilution of 1:100 when tested against purified SSA, SSB, SCL, Sm, Sm/RNP, Jo-1, mitochondrial or ribosomal antigens in an ELISA system designed to detect IgG antibody subclasses
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2ml
Host：human
Source Antibody：human
Grade：Purified
Purity：Fractionated from delipidated, defibrinated and precipitated human serum.
Form：Supplied as a powder lyophilized from a solution containing PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Specificity：Recognizes human Myeloperoxidase (MPO, pANCA) Species Crossreactivity: Should detect MPO from most species. Does not recognize PR-3, SS-A, SS-B, Sm, Sm/RNP, SCL and Jo-1 antigens at a 1:1000 dilution in ELISA.
Isotype：IgG
Calc Applications Abbrev：E IF
Calc Crossreactivity：Hu
Immunogen：Myeloperoxidase (MPO). Autoantibody derived from donor human serum.
