Proteinase 3 (PR3), a constituent of azurophil granules of neutrophils (polymorphonuclear cells, PMNs), is the target antigen for most anti-neutrophil cytoplasmic antibodies (c-ANCA) in Wegener’s Granulomatosis (WG).
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100Optimal dilutions to be determined by the researcher.
Autoantibody Activity:Anti-Proteinase 3 Activity by ELISA: Current lot value: 85EU/mlMPO: 0EU/mlInterpretation of Results:Negative: <0.8EU/ml, Equivocal: 0.8-1.19EU/ml, Positive: >1.2 EU/ml
Other Autoantibody Activity in the Serum:The serum exhibits no reactivity at a dilution of 1:100 when tested against purified SSA, SSB, SCL, Sm, Sm/RNP, Jo-1, mitochondrial or ribosomal antigens in an ELISA system designed to detect IgG antibody subclasses
AntigenResult (EU/ml)SSA2 SSB0SCL0Sm0Sm/RNP3Jo-16
Interpretation of Results:Negative: <16EU/ml, Equivocal: 16-20EU/ml, Positive: >20EU/ml
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2ml
Host：human
Source Antibody：human
Grade：Purified
Purity：Purified by fractionation of delipidated and defibrinated donor human serum.
Form：Supplied as a lyophilized powder from 0.01M PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Specificity：Recognizes human Proteinase 3. Exhibits a C-ANCA pattern by immunofluorescence assay.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：This product is an autoantibody derived from human serum.
