84-1014-06　Ribosomal P 2ml　R2031-25

特徴

• The Ribosomal P antigen consists of three proteins of the 60S ribosomal subunit.
• These three proteins are designated PO (38kD), P1(19kD), and P2(17kD).
• Studies have suggested that one P1 homodimer and one P2 homodimer are attached to PO by the NH2-termini.
• P1 and P2 are believed to be the eukaryotic equivalent of the E.
• coli ribosomal protein L12 and have been shown to contain sequences that are highly conserved among eukaryotes.
• The major immunoreactive epitope of these ribosomal antigens has been mapped and is localized to the carboxy terminus.
• PO, P1, and P2 have an identical 17 amino acid carboxy terminus.
• The Ribosomal P antigens have GTPase activity and have been shown to be phosphorylated.
• Applications:Suitable for use in ELISA and Immunodiffusion.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:100Optimal dilutions to be determined by the researcher.
• Titer:Immunodiffusion: 1:4
• Sm/RNP: Autoantibody Activity (by ELISA):19EU/ml (lot-specific; value may differ from lot to lot).
• Other Autoantibody Activity in the Serum (by ELISA):SSA: 4EU/mlSSB, SCL: 2EU/mlSm: 1EU/ml Sm/RNP: 19EU/ml Jo-1: 3EU/ml
• Interpretation of ELISA Results: <16 EU/ml: Negative; 16-20EU/ml: Equivocal; Positive; >20 EU/ml
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.