Applications:Suitable for use in ELISA, Immunodiffusion and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100Optimal dilutions to be determined by the researcher.
Titer:Immunodiffusion: 1:8
SSB: Autoantibody Activity (by ELISA):763EU/ml (lot-specific; value may differ from lot to lot).
In an ELISA system designed to detect all IgG subclasses, the following purified antigens (dilution of 1:100) responded as:AntigenResult (EU/ml)SSA1825 SSB763SCL2.6Sm1.5Sm/RNP2.6Jo-11.9
Interpretation of Results:<16 EU/ml: Negative16-20 EU/ml: Equivocal>20 EU/ml: Positive
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2ml
Host：human
Source Antibody：human
Grade：Purified
Purity：Purified by delipidation, defibrination and ammonium sulfate precipitation.
Form：Supplied as a lyophilized powder from PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Specificity：Recognizes human La/SS-B. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. Crossreactivity: SSA. No reactivity at a dilution of 1:100 when tested against purified Scl-70, Sm, Sm/RNP and Jo-1 antigens in an ELISA system designed to detect all IgG subclasses.
