The Ro/SSA antigen is comprised or an acidic 60kD protein that may also be associated with a RNA ranging in size from 80 to 112 bases.
The antigenic activity of the Ro/SSA antigen appears to be independent of the RNA since the precipitin activity remains after treatment with RNase or separation from the RNA.
Anti-Ro/SSA autoantibodies were described originally as precipitating autoantibodies in sera of Sjogren's Syndrome and Systemic Lupus Erythematosus patients.
The presence of autoantibodies against the Ro/SSA antigen may be used as a diagnostic aid.
Elevated levels of Anti-Ro/SSA have been detected in as high as 96% of Sjogren's Syndrome patients, 49% of patients with Systemic Lupus Erythematosus, 83% of mothers of infants with isolated complete congenital heartblock or Neonatal Lupus Dermatitis, and over 75% of patients with Subacute Cutaneous Lupus.
Recently, a strong correlation between low positive levels of Anti-Ro/SSA and subdinical dry eyes or mouth was found in a substantial portion of an elderly population.
In addition, it has been shown that 70% of patients with Ro/SSA precipitating antibodies also have rheumatoid factor.
The titer of rheumatoid factor and the concentration of Anti-Ro/SSA have been closely correlated in Primary Sjogren's Syndrome.
Recent developments in ELISA methodology have increased the sensitivity and the ability of the diagnostician to quantitate patient antibody titers.
Applications:Suitable for use in ELISA and Immunodiffusion.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100Optimal dilutions to be determined by the researcher.
Titer:Immunodiffusion: 1:4
Ro/SSA Autoantibody Activity (by ELISA):6447EU/ml (lot-specific; value may differ from lot to lot).
Other Autoantibody Activity in the Serum (by ELISA):SSB: 3.34EU/ml SCL: 3.17EU/mlSm: 2.55EU/ml Sm/RNP: 2.74EU/ml Jo-1: 2.97EU/ml
Interpretation of ELISA Results:Negative: <16EU/ml, Equivocal: 16-20EU/ml, Positive: >20EU/ml
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2ml
Host：human
Source Antibody：human
Grade：Purified
Purity：Purified by fractionation of delipidated and defibrinated donor human serum.
Form：Supplied as a lyophilized powder from PBS (0.01M phosphate/0.13M sodium chloride), pH 7.2, 0.02% sodium azide. Reconstitute with 2ml ddH2O.
Specificity：Recognizes human Ro/SS-A Antigen. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. This product shows no reactivity at a dilution of 1:100 when tested against purified La/SS-B, Sm, Scl-70, Sm/RNP, Jo-1, Mitochondrial or Ribosomal P antigens in an ELISA system designed to detect IgG antibody. Species Crossreactivity: bovine
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Bo Hu
Immunogen：This product is Ro/SSA autoantibody derived from human serum.
