Applications:Suitable for use in FLISA (Fluorescent), Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:FLISA (Fluorescent): 1:10,000-:50,000IF Microscopy: 1:1,000-1:5,000Flow Cytometry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Fluorochrome/Protein Ratio:2.6 moles FITC per mole of goat IgG F(ab’)2
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Prepared by affinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Labeled with FITC (MW390D). Reconstitution: Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol for long term storage.
Specificity：Recognizes Human IgG F(c) fragment. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, human IgG, human IgG F(c) and human serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c), human IgG F(ab’)2 or bovine, equine, mouse, rabbit, rat and sheep serum proteins.
