Lyn is a member of the src family of tyrosine kinases responsible for the regulation of cell signaling mechanisms.
Lyn’s function has been studied in cells of hematopoetic origin where it is associated with the high affinity IgE receptor in basophils and with the p120 Ras-GAP in thrombin-stimulated platelets.
Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells.
Two tyrosyl residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family: autophosphorylation of tyrosine 396 (equivalent to tyrosine 416 of c-Src) located in the catalytic domain correlates with enzyme activation, while Csk-mediated phosphorylation of the C-terminal tyrosine 507 (equivalent to tyrosine 527 of c-Src) inactives the kinases.
Knockout mice have shown that the net effect of Lyn deficiency is to render B cells hyperactive to BCR stimulation, suggesting that the most critical role for Lyn in vivo is in the down-modulation of B cell responses.
Lyn is also involved in controlling the migration and development of specific B cell populations.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:100Immunoprecipitation: 1:200Optimal dilution determined by the researcher.
MW: 56kD
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ug
Host：sheep
Grade：Highly Purified
Purity：Purified Ig fraction
Form：Supplied as a liquid in PBS, pH 7.5
Specificity：Recognizes Lyn as a 56kD protein expressed in B-cells and some lymphoid calls
