CIITA and Regulatory Factor X5 (RFX5) are two transcriptional activators identified in human patients with bare lymphocyte syndrome.
They bind to "X boxes" in class II MHC gene promoters.
X boxes are conserved among class II MHC genes in humans and mice.
The X box sequence is 5'-GTNRCC(N 0-3 )RGYAAC-3' where N is any nucleotide, R is a purine, and Y is a pyrimidine.
Patients with bare lymphocyte syndrome have no class I or class II MHC molecules due to a complete lack of transcription of class I and class II MHC genes.
Both class I and class II MHC genes are flanked by 5’ promoter sequences, which are bound by specific transcription factors.
Consequences of Bare Lymphocyte Syndrome are:an absence of cellular and humoral T cell immune responses.
Applications:Suitable for use in ELISA, Western Blotting, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:2000Western Blot: 1:1000-1:2000Optimal dilutions to be determined by the researcher
Note: Do not supplement buffers with Tween-20 detergent.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add glycerol (25–50%) and freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by ion exchange chromatography.
Form：Supplied as a sterile filtered liquid in PBS, pH 7.2, 0.015M sodium chloride, 0.01% sodium azide.
Specificity：Monospecific for CIITA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to a region near the C-terminal end (aa726-742) of the human CIITA gene conjugated to KLH. Sequence: CGEIKDNKELPQYLALTTR
