Applications:Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy and antibody based assays using streptavidin or avidin conjugates.
Recommended Dilution:Capture ELISA: 1:6000-1:25,000 of the reconstitution concentration is suggested
Storage and Stability:Store vial at 4° C prior to restoration.
Restore with 1ml of deionized water (or equivalent).
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at-20° C or below.
Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4° C as an undiluted liquid.
Dilute only prior to immediate use.
Expiration date is one (1) year from date of restoration.
仕様
Size：2mg
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：≥ 99% Purified by Rat IgG-agarose affinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, rat IgG and rat serum. Biotinylated with biotinamidocaproate N-hydroxysuccinimide ester (BAC).Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.01% thimerosal.
Specificity：Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, rat IgG and rat serum and rat IgG F(ab’)2. No reaction was observed against rat IgG F(c).
