Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration.
Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI).
Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle.
When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I.
The letter “I” is given due to its inhibitory character.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA: Use as a capture
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken-HRP Rb x Ch I1906-12IgY, Chicken-FITC Rb x Ch I1906-14IgY, Chicken-AP Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：chicken
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in TBS, pH 7.4, 0.09% sodium azide
Specificity：Recognizes human Troponin I (Cardiac).
