Protein A is a highly stable surface receptor produced by Staphylococcus aureus, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987).
Each protein A molecule can bind 2 molecules of IgG, allowing the formation of a precipitate (Sjöholm, 1975).
ApplicationsSuitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: > 5000 assays/mg
Optimal dilutions to be determined by the researcher.
Suggested Secondary Antibodies:I1906IgY, Chicken Pab Rb x ChI1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x ChI1906-14IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：chicken
Grade：Highly Purified
Purity：Purified by solvent extraction and column chromatography.
Form：Supplied as a liquid in Tris buffer, pH 7.4, 0.025% sodium azide.
