Coagulation factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF) and is rapidly converted into VIIa.
The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and also has important coagulation-independent functions such as angiogenesis.
The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor.
After cleavage of a signal peptide (residues 1 to 38), the mature chain can be further processed into the light chain (residues 39 to 190) and the heavy chain (residues 191 to 444).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 10ug/ml Western Blot: 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：sheep
Source Antibody：human
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a liquid, 50% glycerol, H2O.
Specificity：Recognizes human Factor VII and VIIa. Will react in Western Blot to both reduced and nonreduced forms of the protein. No reactvity with porcine or mouse Factor VII.
