Factor V is a large, single chain, plasma glycoprotein which is an essential component in the blood coagulation cascade.
During coagulation, the procofactor, factor V, is converted to the active cofactor, factor Va, via limited proteolysis by the serine protease a-thrombin, and less efficiently by factor Xa.
The active cofactor is composed of an NH2-terminal derived heavy chain (Mr=94,000) and a COOH-terminal derived light chain (Mr=74,000) which remain non-covalently associated in the presence of calcium ions.
Factor Va serves as a cofactor for the serine protease factor Xa.
Factor Va and Xa assemble on a phospholipid surface in a non-covalent and calcium ion-dependent manner, to form the prothrombinase complex.
The prothrombinase complex is responsible for the rapid conversion of the zymogen prothrombin to the active serine protease, a-thrombin.
Assembly of the prothrombinase complex increases the rate at which prothrombin is converted to thrombin by nearly 300,000-fold relative to the rate with factor Xa alone.
Down regulation of the prothrombinase complex is accomplished partly through the inactivation of factor Va by activated protein C.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 10ug/mlWestern Blot: 10ug/mlOptimal dilutions to be determined by the researcher.
Extinction Coefficient:E1% at 1cm, 280nm =14.0
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：equine
Source Antibody：human
Grade：Highly Purified
Purity：>95% by SDS-PAGE
Form：Supplied as a liquid in ddH20, 50% glycerol.
Specificity：Recognizes human Factor V. Crossreactivity: Factor Va and activation products.
