84-1012-46　Transforming Growth Factor alpha (TGFa) 1mg　T8250-10

特徴

• TGF-alpha (transforming growth factor alpha) is a 50aa EGF-related polypeptide expressed in a range of tissues, including skin, pancreas, liver, duodenum, salivary glands, urinary and respiratory systems, and the developing and mature central nervous system (CNS).
• TGF-alpha is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR).
• TGF-alpha is mitogenic for multipotent neural progenitors and is involved in astrogliosis.
• Applications:Suitable for use in Direct ELISA, Immunohistochemistry and Western Blot.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/ml immersion fixed paraffin embedded sections of human astrocytoma, pituitary adenoma, and prostate cancer tissue subjected to Antigen Retrieval Reagent-Basic Neutralization: 0.4-0.8ug/ml in the presence of 3ng/ml.
• Measured by its’ ability to neutralize TGF-a-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line.
• Optimal dilutions to be determined by researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.