Pregnenolone (3beta-hydroxypregn-5-en-20-one) is the first steroid to be derived from cholesterol in the pathway of steroidogenesis, and it is the common precursor for all of the adrenal and gonadal steroids.
Its production occurs in the mitochondrion by cleavage of the C-20 side chain of cholesterol by the P-450SCC enzyme.
Once produced, pregnenolone may be utilized by two pathways of steroidogenesis.
Pregnenolone may either be converted to 17-OH pregnenolone via the enzymatic action of 17-hydroxylase or to progesterone via the enzymatic action of 3beta-hydroxypregn-5-en-20-one.
Elevated pregnenolone levels occur in forms of congenital adrenal hyperplasia (CAH), due to 3beta-hydroxysteroid dehydrogenase deficiency or 17alpha-hydroxylase deficiencies.
Higher levels have also been reported in women with idiopathic hirsutism.
Applications:Suitable for use in RIA and ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Titer:RIA and ELISA: 1:200,000
Affinity Constant:1.69x10e10L/mole
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1000Tests
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a liquid. No preservatives added.
