84-1011-65　Histone, Core 250ug　H5110-10

特徴

• Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis and other conditions.
• The presence of autoantibodies to histones are frequently found in several rheumatic disorders.
• In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3.
• Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3.
• The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity.
• In diploid eukaryotic cells, the chromatin fibers are about 20nm in diameter.
• They consist of two major components in equal amounts, DNA and basic proteins called histones.
• Histones are believed to be regularly arranged in the deep grove of the DNA helix.
• The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible.
• This allows for the supercoiling of the chromatin fibers.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applilcations have not been tested.
• Recommended Dilutions:Immunoprecipitation: 1:20Western Blot: 1:200Optimal dilutions to be determined by the researcher.
• Molecular Weights of Histones:Lysine Rich (H1, f1): ~21,500 Slightly Lysine Rich (H2a, f2a2): 14,004 Slightly Lysine Rich (H2b, f2b): 13,774 Arginine Rich (H3, f3): 15,324 Arginine Rich (H4, f2a1): 11,282
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.