84-1011-53　Helicobacter pylori 100ug　H1840-10

特徴

• Helicobacter pylori is a spiral-shaped, Gram-negative rod approximately 0.5x3.0um in size.
• The catalase-positive organism has 4-6 sheathed flagella attached to one pole which allow for motility.
• Helicobacter pylori lives in the stomach and duodenum (section of intestine just below stomach).
• It has a unique way of adapting in the harsh environment of the stomach.
• The inside of the stomach is bathed in about half a gallon of gastric juice every day.
• Gastric juice is composed of digestive enzymes and concentrated hydrochloric acid, which can readily tear apart the toughest food or microorganism.
• Bacteria, viruses, and yesterday’s steak dinner are all consumed in this deadly bath of chemicals.
• It used to be thought that the stomach contained no bacteria and was actually sterile, but Helicobacter pylori changed that.
• The stomach is protected from its own gastric juice by a thick layer of mucus that covers the stomach lining.
• Helicobacter pylori takes advantage of this protection by living in the mucus lining.
• Once H.
• pylori is safely ensconced in the mucus, it is able to fight the stomach acid that does reach it with an enzyme it possesses called urease.
• Urease converts urea, of which there is an abundant supply in the stomach (from saliva and gastric juices), into bicarbonate and ammonia, which are strong bases.
• This creates a cloud of acid neutralizing chemicals around the H.
• pylori, protecting it from the acid in the stomach.
• The reaction of urea hydrolysis is important for diagnosis of H.
• pylori by the breath test.
• Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.