Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form.
It forms a holoenzyme of ~450kD, consisting of 24 subunits of two types, H (heavy; 21kD) and L (light; 19kD), and is capable of storing up to 4,500 atoms of ferric iron.
Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely.
Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood.
Serum ferritin levels serve as an indicator of the amount of iron stored in the body.
Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria.
As ferritin is an acute-phase reactant, it is often elevated during infection.
Defects in ferritin proteins are associated with several neurodegenerative diseases.
Applications:Suitable for use in ELISA, Radiolabeling and Enzyme conjugation.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Affinity Constant:3x10e10L/mole
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human liver and spleen ferritin (100%). Very little crossreactivity to human heart ferritin (<1%).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Affinity pure ferritin from human liver.
