Erythropoietin, normally produced in the kidney and liver, is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
EPO is indicated for treatment of neutropenia or anemia secondary to ZDV- or Ganciclovir-induced bone marrow suppression (most effective in patients with endogenous EPO levels < 500IU/ml).
Applications:Suitable for use in Direct ELISA, Western Blot, Neutralization and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml Recombinant human Erythropoietin
Neutralization: Measured by its the ability to neutralize Eythropoietin-induced proliferation in the TF-1 human erythroleukemic cell line (1).
The ND50 is typically < 3ug/ml in the presence of 0.2units/ml of recombinant human Erthyropoietin (tissue culture grade)
Immunohistochemistry: 5-15ug/ml for immersion fixed paraffin-embedded sections of human kidney cancer tissue
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography. Endotoxin: ≤0.1EU/ug (LAL)
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
Specificity：Recognizes human Erythropoietin.
Isotype：IgG
Calc Applications Abbrev：E IHC N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Erythropoietin expressed in CHO cells.
