The receptors for the endothelins are found on various tissues including lung and intestine.
ETA receptor has been reported in a variety of tissues including placenta, uterus, testis, heart, adrenal gland, lung, kidney, ovary, skin, liver, bladder, large blood vessels, cerebellum, neurons, and meningioma tumor cells.
A splice variant has been identified in melanoma (Zhang et al., 1998).
ESTs have been isolated from libraries from the above-mentioned tissues.
Applications:Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Suitable for paraffin embedded tissue
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ug
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.5. No preservative added.
Specificity：Recognizes rat Endothelin A Receptor at ~39kD. Tested using endothelin A receptor transfected COS cells for the expression of the 39kD segment of the cytoplasmic region of the receptor. Species Crossreactivity: human, mouse and sheep.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt Sh
Immunogen：Synthetic peptide corresponding to aa410-422, QEQNHNTERSSHK of rat Endothelin A Receptor (C-Terminal-KLH). MW: 39kD.
