coli) is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms).
Most E.
coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls.
The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, or by preventing the establishment of pathogenic bacteria within the intestine.
E.
coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination.
The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of metagenics, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology.
E.
coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885, and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.
Applications:Suitable for use in FLISA and Immunofluorescence.
Other applications have not been tested.
Recommended Dilutions:Immunofluorescence: Acetone fixation of the antigen is recommended
Optimal dilutions to be determined by the researcher.
Titer:>1:2000 by ELISA vs Serotypes: O157:H7, O20, O125, O55, O111 and K12
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：rabbit
Grade：Purified
Purity：Purified by chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes all O and K antigenic serotypes of E. coli. Note: This antibody is unabsorbed and will cross-react with related Enterobacteriaceae.
