Human Chorionic Gonadotropin (HCG) is a glycoprotein, which is secreted in large quantities by normal trophoblasts.
It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy.
HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha- and beta-subunits.
The alpha-subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).
A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP), and HCG but negative for EMA and AFP is probably a choriocarcinoma.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1:350,000 (Titer)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1ml
Host:rabbit
Source Antibody:human
Grade:Serum
Purity:Neat serum
Form:Supplied as a liquid. No preservatives added.
Specificity:Recognizes human Chorionic Gonadotropin, alpha: 100%.
Isotype:IgG
Calc Applications Abbrev:E
Calc Crossreactivity:Hu
Immunogen:Purified human Chorionic Gonadotropin, alpha