Chlamydia is caused by the bacterium Chlamydia trachomatis.
The intracytoplasmic inclusions caused by the bacterium are draped around the infected cell’s nucleus.
Chlamydia has a genome size of approximately 500-1000 kilobases and contains both RNA and DNA.
The organism is also extremely temperature sensitive and must be refrigerated at 4°C as soon as a sample is obtained.
Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia.
It persists at body sites that are inaccessible to phagocytes, T-cells and B-cells.
It also exists as 15 different serotypes.
These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3).
Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes.
The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall.
This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it.
It may, however, contain a carboxylated sugar other than muramic acid.
The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds.
It also contains cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan.
This unique structure allows for intracellular division and extracellular survival (Hatch 1996).
Kenneth Todar, University of Wisconsin-Madison
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested
Recommended Dilutions:ELISA: 1:1000Immunohistochemistry: 1:200Western Blot: 1:200Optimal dilutions to be determined by researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：goat
Grade：Purified
Purity：Purified IgG fraction.
Form：Supplied as a liquid in PBS, 1% BSA, 0.002% thimerosal. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes Major Outer membrane Protein (MOMP) of Chlamydia trachomatis.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Immunogen：Purified Major Outer Membrane Protein (MOMP) from strain L2
